Enzymatic Hydrolysis

 15 days- March 2000

 A Lipase is a water-soluble enzyme that catalyzes the hydrolysis of ester bonds in water–insoluble, lipid substrates. Most lipases act at a specific position on the glycerol backbone of a lipid substrate (A1, A2 or A3).. A myriad of other lipase activities exist in nature, especially when the phospholipases and sphingomyelinases are considered.

It was then interseting to use lipases as regioselective tools for chemical transformations. On a substrate containing ester and phosphoester groups, a variety of lipases, extracted from different organisms was screened, using a colometric test. When the suitable lipase was selected, a study of NMR spectra began in order to determine if the hydrolysis was chimioselective (phosphosters vs esters) and regioselective

T. Lalot, E. Marechal, J.P. Vairon

« Macromolecular Synthesis »,

UMR 7610, Université Pierre et Marie CURIE,11 Place Jussieu, Paris, FRANCE