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- Preparation of the starting materials
- Design
and manufacture of a versatile setup of
pattern photobleaching using evanescent
waves
- Synthesis
and purification of various transmembrane
peptides ( with R.S. HODGES, CO, USA)
(click here for details)
- Design
of a phase of bilayers for protein
lateral diffusion and tests of cristallisation
- Design
and manufacture of microfluidics chips (
with T. THORSEN, MIT, Boston, USA)
- labeling
and reconstitution of transmembrane proteins
in Giant Unilamellar Vesicles (
list of the techniques here)
I
- Processes of diffusion :
II
- Lateral mobility in membranes (click
here for details)
- Dependence
in Radius of the protein, using transmembrane
peptides and proteins : OmpA, OprM, and
BR
- Importance
of the viscosity of the bounding fluid :
effects of sugars, dextran, or PEGs on membranes.
- Weight
of the hydrophobic thickness of the membrane
- Proposal
of an heuristic law; possible theoritical
model ( with N. GOV, Israel)
III
- Understanding the anchorage of transmembrane
peptides using single-molecule pulling experiments
(click
here)
- Role
played by the hydrophobicity of the helix
- Importance
of the polar heads
- Modelisation
of the energy barriers explored by the peptides
during their extraction
- Deformation
of membranes and threshold for microtubules
formation;
- dependence
force-speed of extraction for the tubes
IV
- Diverse consequences of the hydrophobic
mismatch : (to
learn more...)
A)
On the individual level :
- anchorage
of transmembrane peptides : the longer
the stronger ?
- Asymetric
impact on lateral diffusion: "tilting"
and "pinching" behaviors
- Conformation
of peptides as determined by IR Spectroscopy
( with F. HOMBLE, Belgium)
- Determination
of the critical mismatch for peptide insertion
B)
Collective behavior controlled by mismatch :
- auto-association
of peptides in micro-patches for a critical
mismatch
- giant
patches (up to 100 µm) and spontaneous formation
of vesicles
V
- characterization of protein-protein interactions
- Clustering
of Bacteriorhodopsin upon photoactivation
( with P. BASSEREAU, Institut Curie, Paris,
France)
- Interaction
between the Snares proteins VAMP- SNAP25
- Syntaxin (D. TARESTE, J. ROTHMAN, NY,
USA)
- Comparaison
of the diffusion of CX3CR1 and CCR5 proteins
in cells ( with P. DETERRE, Pitie-Salpetriere
Hospital, Paris, France.)
VI
- Cristallisation of transmembrane proteins
- Tests
of isotropic, cubic-like phases of bilayers
- Tests
of progressive variation using microfluidics
chips
In
collaboration with A. DUCRUIX, laboratoire de
Cristallographie, Universite de Pharmacie, Paris.
If you want to know
more, please let me know and I'd be happy to send you
preprints or drafts...
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