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Competences acquired during my formation :
· PEPTIDES : Synthesis and purification of hydrophobic, transmembrane peptides (diblocs and triblocs.) (training in collaboration with R.S. Hodges, Canada.)
· PROTEINS : Labeling and reconstitution of membrane proteins into Giant Unilamellar Vesicles (GUVs). (electroformation techniques). Micromanipulation and Langmuir-Blodgett techniques.
· FRAPP : Measure of lateral mobility of peptides and proteins embedded in various bilayers (lipids or model bilayers of tunable thickness.) Characterization of diffusive processes.
· SINGLE MOLECULE : Single-molecule pulling experiments: during my Ph.D, I measured the strength of anchorage of various transmembrane peptides incorporated in GUVs.
· IR SPECTROSCOPY was performed on oriented bilayers ( in collaboration with F. Homble, Belgium) to characterize tilt angles of peptides under hydrophobic mismatch.
· MODEL MEMBRANE : Use of lipids or surfactants to create various phase of bilayers (cubic, sponge or lamellar phase), characterized by SAXS and DLS/DQLS experiments. The transmembrane proteins are easily incorporated in these model systems and protein-protein interactions can be precisely studied. These phases of multi-connected bilayers are tested for membrane protein CRYSTALLOGRAPHY too.
· MICROFLUIDICS : Design and manufacture of microfluidic networks using multilayer soft lithography; valves, pumps and micro-optics can be integrated on the micro-chips.
· OPTICS : Conception and realization of optical setups (pattern photobleaching using 4 laser beams to measure the mobility of particles in evanescent waves); manufacture of mechanical components. I particularly worked on news techniques of molding, using surface tension, to produce micro-optics that can be assembled into a microscope.
Copyright(c) 2005 - see yann@gambin.com
gambin@lps.ens.fr