Cours 3

[Enhanced diffusion][models of anomalous diffusion][Peptide synthesis][lateral mobility in membranes][single molecule pulling experiments][consequences of hydrophobic mismatch][Techniques used]

Biomembrane Force Probe

Understanding the anchorage of transmembrane peptides using single-molecule pulling experiments

We synthesized 7 different peptides, made of a hydrophobic alpha-helix caped on one or both sides by polar heads. These peptides were incorporated into GUVs of SOPC, and one-by-one extracted from the membrane.

Using the Biomembrane Force Probe technique sketched above, we measured the strength of anchorage at the single-molecule level; we successively studied :

the role played by the hydrophobicity of the helix

the importance of the polar heads,

the impact of hydrophobic mismatch and local deformations.

Our data will help us to draw a general model of the energy barriers explored by the peptides during their extraction.

In addition, we studied the deformation of membranes and threshold for microtubules formation, as well as force-speed dependence during their extraction.

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